Resumen: Chicken lymphocyte membrane immunoglobulins (Ig), were precipitated with mouse monoclonal antibodies specific for heavy and light chain isotypes and analyzed by polyacrylamide gel electrophoresis. Very little or no membrane-bound IgG and IgA was detected. After sequential precipitation and removal of IgM reactive with any of three monoclonal anti-mu antibodies, anti-light chain antibody precipitated residual Ig with a relative electrophoretic mobility similar to that of IgM. Under reducing conditions, these surface Ig molecules had a heavy chain that appeared slightly larger (approximately 81,000 daltons) than mu-chain (approximately 79,000 daltons), and light chains of approximately 25,000 daltons. Complete clearance of membrane-bound IgM reactive with an anti-mu allotype antiserum left similar molecules precipitate by monoclonal anti-light chain antibody. These non-IgM molecules could be detected on the surface of lymphocytes from blood, spleen, bursa and the B cell line RAV-1, but not from thymus or blood from an agammaglobulinemic chicken. After capping of B cell surface IgM with anti-mu, immunofluorescent staining with anti-light chain antibody revealed residual Ig molecules disturbed across the surface of more than 90% of the IgM-bearing cells. The data suggest the existence of an avian homologue of mammalian IgD. Affinity-purified goat anti-mu antibodies and a fourth monoclonal anti-mu antibody reacted with both IgM and the putative IgD molecules, which suggests that the IgD homologue shares at least one common determinant with chicken IgM.

Autor/es: Chen CL - Lehmeyer JE - Cooper MD

Publicado en: J Immunol (129(6):2580-5, 1982 Dec.)

ISSN: 0022-1767

Tipo/s de publicación/es: JOURNAL ARTICLE - RESEARCH SUPPORT, U.S. GOV'T, P.H.S.

Año de publicación: 1982

País: Estados Unidos

Idioma/s: Inglés

Descriptor/es: Animales - Anticuerpos Monoclonales - Especificidad de Anticuerpos - Pollos - Epitopes - Inmunoglobulina D - Linfocitos - Peso Molecular - Receptores del Antígeno de la Célula B

Substancia/s: 0 (Antibodies, Monoclonal) - 0 (Epitopes) - 0 (Immunoglobulin D) - 0 (Receptors, Antigen, B-Cell)